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dc.contributor.authorRounge, Trine Ballestad
dc.contributor.authorFuru, Kari
dc.contributor.authorSkotheim, Rolf Inge
dc.contributor.authorHaugen, Trine B.
dc.contributor.authorGrotmol, Tom
dc.contributor.authorEnerly, Espen
dc.date.accessioned2017-05-04T10:50:12Z
dc.date.accessioned2017-05-10T12:05:55Z
dc.date.available2017-05-04T10:50:12Z
dc.date.available2017-05-10T12:05:55Z
dc.date.issued2015
dc.identifier.citationRounge TB, Furu K, Skotheim RI, Haugen TB, Grotmol T, Enerly E. Profiling of the small RNA populations in human testicular germ cell tumors shows global loss of piRNAs. Molecular Cancer. 2015;14(153)language
dc.identifier.urihttp://hdl.handle.net/10642/4921
dc.description.abstractBackground Small non-coding RNAs play essential roles in gene regulation, however, the interplay between RNA groups, their expression levels and deregulations in tumorigenesis requires additional exploration. In particular, a comprehensive analysis of microRNA (miRNA), PIWI-interacting RNAs (piRNAs), and tRNA-derived small RNAs in human testis and testicular germ cell tumor (TGCT) is lacking. Results We performed small RNA sequencing on 22 human TGCT samples from 5 histological subtypes, 3 carcinoma in situ, and 12 normal testis samples. miRNA was the most common group among the sequences 18–24 nt in length and showed histology-specific expression. In normal samples, most sequences 25–31 nucleotides in length displayed piRNA characteristics, whereas a large proportion of the sequences 32–36 nt length was derived from tRNAs. Expression analyses of the piRNA population demonstrated global loss in all TGCT subtypes compared to normal testis. In addition, three 5′ small tRNA fragments and 23 miRNAs showed significant (p < 10−6) differential expression in cancer vs normal samples. Conclusions We have documented significant changes in the small RNA populations in normal adult testicular tissue and TGCT samples. Although components of the same pathways might be involved in miRNA, piRNA and tRNA-derived small RNA biogenesis, our results showed that the response to the carcinogenic process differs between these pathways, suggesting independent regulation of their biogenesis. Overall, the small RNA deregulation in TGCT provides new insight into the small RNA interplay.language
dc.language.isoenlanguage
dc.publisherBioMed Centrallanguage
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.language
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectpiRNAlanguage
dc.subjectTesticular cancerlanguage
dc.subjectmiRNAlanguage
dc.subjectRNAlanguage
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762language
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk molekylærbiologi: 711language
dc.titleProfiling of the small RNA populations in human testicular germ cell tumors shows global loss of piRNAslanguage
dc.typeJournal articlelanguage
dc.typePeer reviewedlanguage
dc.date.updated2017-05-04T10:50:11Z
dc.description.versionpublishedVersionlanguage
dc.identifier.doihttp://doi.org/10.1186/s12943-015-0411-4
dc.identifier.cristinID1278554
dc.relation.projectIDNorges forskningsråd: 179571
dc.source.issn1476-4598


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.