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dc.contributor.authorKase, Eili Tranheimen_US
dc.contributor.authorNikolić, Natašaen_US
dc.contributor.authorBakke, Siril Skareten_US
dc.contributor.authorBogen, Kaja Kamillaen_US
dc.contributor.authorAas, Vigdisen_US
dc.contributor.authorThoresen, G. Hegeen_US
dc.contributor.authorRustan, Arild Christianen_US
dc.date.accessioned2013-08-09T08:46:37Z
dc.date.available2013-08-09T08:46:37Z
dc.date.issued2013-04-01en_US
dc.identifier.citationKase, E. T., Nikolić, N., Bakke, S. S., Bogen, K. K., Aas, V., Thoresen, G. H., & Rustan, A. C. (2013). Remodeling of oxidative energy metabolism by galactose improves glucose handling and metabolic switching in human skeletal muscle cells. PloS one, 8(4), e59972.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.otherFRIDAID 1021626en_US
dc.identifier.urihttp://hdl.handle.net/10642/1555
dc.description.abstractCultured human myotubes have a low mitochondrial oxidative potential. This study aims to remodel energy metabolism in myotubes by replacing glucose with galactose during growth and differentiation to ultimately examine the consequences for fatty acid and glucose metabolism. Exposure to galactose showed an increased [14C]oleic acid oxidation, whereas cellular uptake of oleic acid uptake was unchanged. On the other hand, both cellular uptake and oxidation of [14C]glucose increased in myotubes exposed to galactose. In the presence of the mitochondrial uncoupler carbonylcyanide p-trifluormethoxy-phenylhydrazone (FCCP) the reserve capacity for glucose oxidation was increased in cells grown with galactose. Staining and live imaging of the cells showed that myotubes exposed to galactose had a significant increase in mitochondrial and neutral lipid content. Suppressibility of fatty acid oxidation by acute addition of glucose was increased compared to cells grown in presence of glucose. In summary, we show that cells grown in galactose were more oxidative, had increased oxidative capacity and higher mitochondrial content, and showed an increased glucose handling. Interestingly, cells exposed to galactose showed an increased suppressibility of fatty acid metabolism. Thus, galactose improved glucose metabolism and metabolic switching of myotubes, representing a cell model that may be valuable for metabolic studies related to insulin resistance and disorders involving mitochondrial impairments.en_US
dc.language.isoengen_US
dc.publisherPublic Library of Science (PLOS)en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Farmakologi: 728en_US
dc.titleRemodelling of oxidative energy metabolism by galactose improves glucose handling and metabolic switching in human skeletal muscle cellsen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionCopyright: 2013 Kase et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
dc.identifier.fulltextfulltext https://oda.hio.no/jspui/bitstream/10642/1555/1/1021626.pdf
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0059972


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